Improved preparation of buffy-coat-derived platelet concentrates and factors affecting platelet recovery

doi:10.3969/j.issn.2095-4344.2014.07.017

Abstract

Abstract:

BACKGROUND: Buffy-coat-derived platelet concentrates and plasma-rich platelet concentrates have a high incidence of invalid infusion and adverse reactions.
OBJECTIVE: To observe the improved preparation of buffy-coat-derived platelet concentrates and to analyze the influential factors relevant to platelet recovery.
METHODS: 400 mL of blood sample extracted from 126 cases were randomly divided into improved buffy-coat group, buffer-coat group and platelet-rich plasma after 4-6 hours. The 3-step centrifugal method was used for improved preparation of buffy-coat-derived platelet concentrates: step 1, centrifugation at 2 300 r/min for 12 minutes at (22±2)℃ with a deceleration of 5; step 2, centrifugation at 910 r/min for 10 minutes at (22±2)℃; step 3, centrifugation at 2 800 r/min for 12 minutes at (22±2)℃. After centrifugation, the upper layer containing few platelets was removed, and the rest 30 mL platelet suspension was platelet concentrates. Factors affecting platelet recovery were analyzed through literature retrieval.
RESULTS AND CONCLUSION: There was no difference in platelet number among the three groups before preparation of platelet concentrates (P > 0.05). A higher rate of platelet recovery was found in the platelet-rich plasma group and improved buffy-coat group compared with the buffy-coat group (P < 0.05), but there was no difference between the former two groups (P > 0.05). There were less residual red blood cells and white blood cells in the two buffy-coat groups than the platelet-rich plasma group (P < 0.05), but there was no difference between the two buffy-coat groups (P > 0.05). The recovery rate of prepared platelet concentrates was affected by the whole blood amount, centrifugal speed, centrifugation time and methods. Improved buffy-coat method for preparation of platelet concentrates can be generalized in blood centers or blood stations, because it can reduce residual red blood cells and white blood cells and increase rate of platelet recovery.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: platelets, platelet count, blood transfusion, leukocytes, erythrocytes

CLC Number:

R318

Shan Hong, Wang Jiao-jie, Bie Li-li, Liu Min, Qi Zheng, Duan Yan-li. Improved preparation of buffy-coat-derived platelet concentrates and factors affecting platelet recovery[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(7): 1082-1087.

Figures/Tables 1

References

[1] 陈方祥,张彤,王荣,等.血小板制备与输注研究近况[J].国外医学输血及血液学分册,1996,5:304.

[2] 禹晓彬,李锡兰.白膜层静置时间对浓缩血小板回收率的影响[J].国际医药卫生导报,2013,19(22):3462-3464.

[3] 李小梅,梁芬,符雪丽,等. 探讨手工制备浓缩血小板的影响因素[J].中国现代医药杂志,2007,9(5):78-79.

[4] 唐泽萍.手工制备浓缩血小板影响因素探讨[J].西部医学,2012, 24(10):2000-2001.

[5] 张玉红,李建斌,单泓.富浆法制备浓缩血小板影响因素分析[J].河南医学研究,2012,21(3):355-357.

[6] Hux BD, Martin LG.Platelet transfusions: treatment options for hemorrhage secondary to thrombocytopenia. J Vet Emerg Crit Care (San Antonio). 2012;22(1):73-80.

[7] 人民共和国国家质量监督检验检疫总局发布.中华人民共和国国家标准《献血者健康检查要求》(GBl8467-2001)[S].北京, 2001:1.

[8] 尹涛,白兰兰,石丽萍,等.三联袋处理血小板冲红方法的建立[J].中国输血杂志,2011,24(4):354-355.

[9] Schrezenmeier H, Seifried E.Buffy-coat-erived pooled platelet concentrates and apheresis platelet concentrates: which product type should be preferred?Vox Sang.2010; 99(1):1-15.

[10] Vassallo RR, Wagner SJ, Einarson M, et al.Maintenance of in vitro properties of leukoreduces whole blood-derived pooled platelets after a 24-hour interruption of agitation. Transfusion.2009;49(10):354-355.

[11] 赵凤绵,张爱红,梁晓虎,等.浓缩血小板的应用现状与进展[J].中国输血杂志,2007,20(1):75-77.

[12] 欧阳锡林,刘景汉,黄宁,等.悬浮红细胞保存过程中高浓度血小板-白细胞聚合物的形成及预防措施[J].解放军医学杂志,2002, 27(12):1096.

[13] Li NL,Goodall AH, Hjemdahl P.Efficient flow cytometric assay for platelet-leukocyte aggregates in whole blood using fluorescence signal triggering.Cytometry.1999; 35(2):154.

[14] 马曙轩,欧阳锡林,刘景汉.手工采集血小板的制备与临床应用[J].中国输血杂志,2004,17(5):373.

[15] 卢发强,赵士刚,杨玉清,等.白膜层过夜放置对汇集手工浓缩血小板聚集反应和体外激活的影响[J].中国输血杂志,2008,21(5):364-365.

[16] 姚根宏,陈虎诚,陈艳丽,等.少量浓缩血小板的手工制备方法建立[J].中国血液流变学杂志,2013,23(2):339-340.

[17] 巴丽聪,于金华.影响手工制备浓缩血小板质量的原因分析[J].中国民康医学,2011,23(24):3126.

[18] 迟镝.血小板贮存袋对手工分离浓缩血小板贮存的影响分析[J].中国社区医师,2012,14(24):174-176.

[19] 何佳玲,石小兰.手工浓缩血小板不合格原因分析[J].中外医学研究,2011,9(15):133.

[20] 王姣杰,李建斌.手工浓缩血小板的细菌污染分析[J].中国医药导报,2009,6(1):142.